Reducing Carbon Input Improved the Diversity of Bacterial Community in Large-Scale Biofloc Shrimp Culture Facilities

In this study, a group of large-scale biofloc shrimp culturing facilities were designed. The bioflocs domesticated by continuously reducing the ratio carbon to feed. bacterial community diversity on 20th, 40th, 60th, and 80th days was analyzed high-throughput sequencing technology. results showed that content (0~16.8 ± 4.3) mL/L, TSS concentration (0~247.46 27.3) total ammonia nitrogen (0~0.28 0.052) mg/L, nitrite (0~4.13 1.42) nitrate (108.57 19.6) mg/L all within safe range Litopenaeus vannamei. With progress input, Chao1 index, number operational taxonomic units, Shannon index increased significantly. OTUs B80 (572.36 13.26) significantly higher than B60 (489.69 12.97), B40 (423.35 18.46) B20 (407.67 15.65) (p < 0.05). (768.58 36.96) (646.8 52.53), (569.7 46.53) (516.3 21.35) (5.63 0.16) (4.85 0.13), (4.68 0.21) (3.65 0.22), with significant difference At end experiment, domestication formed micro-ecosystem Proteobacteria as carrier (46.98 15.82%), Chloroflexi skeleton (2.2 0.36%), Nitrospirae (1.35 0.26%) main water treatment functional bacteria, other bacteria auxiliary phosphorus removal; genus level, unclassified_f_Rhodobacteracea (22.97 3.82%), Ruegeria (10.35 1.26%), Muricauda (5.73 0.61%), Algoriphagus (3.75% 0.85%) Nitrospira (1.56 0.56%) are dominant bacteria. Under synergistic effect above system remains relatively stable. survival rate unit yield (65.32 6.85)% (4.15 1.58) kg/m3, respectively.